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Tobacco Science
and Technology

Tobacco Science
and Technology

Tobacco Science and Technology

Volume 56 , Issue 06

25 Sep 2023

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Volume - 56 , Issue 06

30 Sep 2023

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Tobacco Science and Technology

Community structure and diversity analyses of adnascent bacteria on tobacco seeds

Bacteria and fungi present during pile-fermentation of Sichuan dark tea play a key role in the development of its aesthetic properties, such as color, taste, and fragrance. In our previous study, high-throughput sequencing of dark tea during fermentation revealed Aspergillus was abundant, but scarce knowledge is available about bacterial communities during pile-fermentation. In this study, we rigorously explored bacterial diversity in Sichuan dark tea at each specific stage of piling. Analysis of cluster data revealed 2,948 operational taxonomic units, which were divided into 42 ph

Differences in chemical compositions of flue-cured tobacco stems

Yellowing is a key stage in the curing of flue-cured tobacco (Nicotiana tobacum L.) as much of the chemical transformation occurs during this period. This study examined the effect of different yellowing degrees on the value of flue-cured tobacco leaves at the farm level for both processing and manufacturing. The study was conducted in the counties of Chuxiong, Dali, and Yuxi in Yunnan, China over two years. Yellowing treatments have been designed to have either a mild or a regular yellowing degree. Yield, value, appearance, suction property, smoking characteristics, and physical resistance

An emotion classification method for cigarette consumers' evaluation based on combination of BiLSTM and Attention Mechanism

Since the existing music emotion classification researches focus on the single-modal analysis of audio or lyrics, the correlation among models are neglected, which lead to partial information loss. Therefore, a music emotion classification method based on deep learning and improved attention mechanism is proposed. First, the music lyrics features are extracted by Term Frequency-Inverse Document Frequency (TF-IDF) and Word2vec method, and the term frequency weight vector and word vector are obtained. Then, by using the feature extraction ability of Convolutional Neural Network (CNN) and the

Determination of major chemical components in exhaled aerosol of heated tobacco products in relation to inhaled aerosol

Heated tobacco products (HTPs) and electronic cigarettes (ECs) represent a potentially less harmful alternative to traditional tobacco products, such as conventional cigarettes (CCs), with growing popularity amongst adult smokers worldwide. Their users exhale a very dynamic aerosol to indoor air which undergoes rapid transformations. In the present study, we assessed the dynamics of the generated exhaled aerosols following use of a new HTP (branded as “Pulze”, operating in eco and standard modes) and an e-cigarette (“myblu”) in a chamber environment by three volunteers, controlling

Effects of aging in two types of wooden containers on volatile components in cigars

The aim of the study was to estimate the thiocyanate levels in saliva of cigarette smokers in comparison to e-cigarette smokers and nonsmokers. To improve our understanding of the influence of smoking on the oral level of thiocyanate, we conducted an assessment of human saliva, in 24 individuals (eight tobacco smokers, eight e-cigarette smokers, and eight nonsmokers). High-Performance Liquid Chromatography with ultraviolet detection (HPLC-UV) using a unique phosphatidylcholine column was applied in this assay. Thiocyanate ion was detected directly by its absorbance at 210 nm. The method pre

Analysis on cigarette smokers' saliva components by closed loop stripping

The aim of the study was to estimate the thiocyanate levels in saliva of cigarette smokers in comparison to e-cigarette smokers and nonsmokers. To improve our understanding of the influence of smoking on the oral level of thiocyanate, we conducted an assessment of human saliva, in 24 individuals (eight tobacco smokers, eight e-cigarette smokers, and eight nonsmokers). High-Performance Liquid Chromatography with ultraviolet detection (HPLC-UV) using a unique phosphatidylcholine column was applied in this assay. Thiocyanate ion was detected directly by its absorbance at 210 nm. The method pre

Effects of four biocontrol strains on tobacco black shank control and microorganism community structure of rhizosphere soil

Black shank, caused by the oomycete pathogen Phytophthora nicotianae, is responsible for huge economic losses worldwide. Research has focused on biocontrol to prevent disease and to minimize the use of synthetic fungicides. We explored and compared the efficacy of suppressive microflora cultured from soil and roots on the growth of P. nicotianae for controlling the incidence of black shank. We found that 31 microfloral communities, enriched from 40 root samples but only 18 microfloral communities from soil samples, were antagonistic to P. nicotianae. In the field experiment, the root functi

Effects of overexpression of PnSS gene from Panax notoginseng on tobacco terpenoid contents and plant growth and development

Nicotiana tabacum solanesyl diphosphate synthase 1 (NtSPS1) is the key enzyme in solanesol biosynthesis. However, changes in the solanesol content, plant growth, photosynthesis, and metabolome of tobacco plants after NtSPS1 overexpression (OE) have not been previously reported. In the present study, these parameters, as well as photosynthetic gas exchange, chlorophyll content, and chlorophyll fluorescence parameters, were compared between NtSPS1 OE and wild type (WT) lines of tobacco. As expected, NtSPS1 OE significantly increased solanesol content in tobacco leaves. Although NtSPS1 OE sign

Air flow distribution in a lit cigarette during puffing

Establishing a realistic gas flow velocity distribution inside a cigarette filter during smoking is important to understand filtration mechanisms of different mainstream smoke species and the overall effect of filter designs on mainstream smoke composition. In this paper, an experimental method is described which directly measures the gas pressure field inside a cellulose acetate filter during cigarette smoking. This was demonstrated by using 3R4F research reference cigarettes smoked under a 35 mL puff of 6 s duration. In addition, filter temperature measurements were also carried out at mu

Migration characteristics of moisture and glycerol in cut tobacco strips during pneumatic drying

Drying is a technique that involves removal of moisture using heat energy. This heat affects the protein components in foods especially the thiosulphide groups, which causes hydrophobic bond break that has been attributed greatly to denaturation during drying. Safe moisture content (SMC) is the extent to which moisture can be withdrawn from food crops during drying that such crops can be considered safe for storage with minimal loss of nutritional qualities. Several reported minimum moisture contents, and bulk nutrients’ levels of crops were collected for the purpose of this review, and s