Abstract : The Anthyllis barba jovis plant is a fabaceae belonging to the Algerian flora. Our work is based their valorization on a physico-chemical analysis of the leaves such as contents (water, dry matter and organic matter), this analytical procedure followed by some biochemical dosages (chlorophyll pigments, proteins, soluble sugars, and polyphenols). An anatomical study was also carried out on two types of samples (leaf and stem) of the plant to be studied The species was harvested at the Soug Rguibette station, in the El Kala National Park (North-East Algeria). Leaves showed a water content of around 59.45 ± 0.97%, while dry matter accounted for 40.55%, a high organic matter content of around 91.13%, and recorded levels of total chlorophylls (Chl T), carotenoids (Car), soluble sugars and proteins in the order of 0.978 ± 0.157 mg/g; 0.415 ± 0.066 mg/g; 10 ± 0.01 mg/g DM and 73.42 ± 1.926 mg/g DM. Polyphenols estimated by the Folin Ciocalteu reagent were found to be around 60.045 ± 0.650 mg Ag/g DM. The anatomical sections in general revealed well-defined morphological and histological characteristics, a small leaf surface, cutinized epidermis, palisading parenchyma on both sides of the mesophyll) and a particularity concerning the presence of non-glandular hairs serving as an adaptation mechanism to climatic conditions.