A field experiment was conducted during the autumn growing season of 2025 at the experimental field of the agricultural extension farm located in the Al-Mahnawiya area of ​​Babylon Governorate. with the aim of evaluating the performance of some maize genotypes and to estimate some of their genetic parameters under the influence of spraying with the biostimulant Biozyme. The experiment was carried out according to a randomized complete block design (RCBD) with a split-plot arrangement and three replications. The main plots included the Biozyme spraying treatments (Distilled water, 1.5 ml L⁻¹, 2.5 ml L⁻¹, and 3.5 ml L⁻¹), while the subplots included six genotypes (Buhouth 5018, Sara, Al-Maha, Fajr1, Baghdad3, and ZP707). The results showed significant differences among Biozyme spraying concentrations. The concentration of 3.5 ml L⁻¹ significantly outperformed the other treatments in plant height, number of leaves, leaf area, and chlorophyll content, with means of 190.2 cm, 13.4 leaves plant-1, 6000.1 cm², and 58.2 SPAD, respectively. In contrast, the concentration of 2.5 ml L⁻¹ showed a significant superiority in the number of rows per ear, with a mean of 17 rows ear-1. The concentration of 1.5 ml L⁻¹ was also observed to be superior in the number of grains per row and the number of grains per ear, with means of 32.7 grains row-1 and 550.1 grains ear-1, respectively. As for the genotypes, the Fajr1 genotype was distinguished in yield traits and components such as the number of grains per row and the number of grains per ear, with a means of 31.8 grains row-1 and 541.0 grains ear-1 respectively. Accordingly, this genotype can be considered one of the economically important genotypes due to its favorable yield performance. Results also indicated significant interaction between the factors with changes in the genotype variations depending Biozyme concentration.