Abstract :
Lavandula stoechas L. is an aromatic plant with widespread distribution, traditionally used in local medicine for its expectorant, antispasmodic, carminative, sedative, anti-inflammatory, and digestive properties. The aerial parts of the plant, harvested at the flowering stage, were extracted with 85% methanol for three days. The resulting suspension was filtered using a Buchner funnel and concentrated under reduced pressure with a rotary evaporator to yield a crude methanol extract (ME). This extract was then partitioned with hexane, chloroform, and ethyl acetate. Each solvent fraction was evaporated to dryness under reduced pressure, resulting in hexane (HE), chloroform (CHE), ethyl acetate (EAE), and remaining aqueous (AqE) extracts. Antioxidant activities of these extracts were evaluated through four in vitro assays: DPPH, hydroxyl radical, ferrous ion chelation, and lipid peroxidation. All extracts exhibited strong radical scavenging and iron-chelating abilities. The ethyl acetate extract demonstrated the highest DPPH scavenging activity (IC50 = 5.56 µg/mL) and hydroxyl radical scavenging activity (72.01 µg/mL). The methanol extract (ME) showed the greatest chelating activity (IC50 = 0.44 mg/mL) and the most effective lipid peroxidation inhibition (95.09%). These findings suggest that the plant is a promising source of natural antioxidants.