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[This article belongs to Volume - 57, Issue - 05]

Abstract : A quantitative investigation was undertaken to explore the diverse applications of Opuntia Ficus-Indica. The study, initiated with a biological examination of seeds, focused on specimens gathered in October 2021 from the Sidi-Fredj area in the Souk-Ahras province of North-East Algeria. Four types of organic extracts: Ethanol, methanol, ethyl acetate, and hexane, underwent quantitative analysis, assessing tannins, flavonoids, and total polyphenols content. Results indicated that the methanolic extract exhibited the highest values for all three components (316; 182.51; and 454.33 µg/mL respectively), followed by ethyl acetate extract (283.38; 118.33 and 409.4µg/mL respectively). Total antioxidant activity, as assessed in vitro, revealed moderate in ethyl acetate and methanol, compared to hexane and ethanol (3.91; 3.56; 3.27 and 3.01µg/mL respectively). Methanol extract exhibited the most potent FRAP iron reduction with a CI50 of 22.09µg/mL, while ascorbic acid showed a CI50 of 1.21µg/mL as a positive control. In the DPPH radical reduction test, ethanol extract recorded a CI50 of 2.19µg/mL, followed by ethyl acetate with an IC50 of 1.93µg/mL. In the beta-carotene bleaching test, significant anti-radical activity was observed in methanol, followed by ethyl acetate (AA = 2.04; 1.82µg/mL respectively). These findings provide valuable insights for future biological investigations concerning this plant commonly known as 'el hindi’. Extract exhibited the highest phenolic contents, followed by the ethyl acetate extract .Total antioxidant activity was moderate in ethyl acetate and methanol compared to hexane and ethanol. The methanol extract showed the most potent FRAP iron reduction. In the DPPH test, the ethanol extract recorded a CI50 of 2.19 µg/mL, followed by ethyl acetate with an IC50 of 1.93 µg/mL. Significant anti-radical activity was observed in methanol, followed by ethyl acetate, in the beta-carotene bleaching test.