Abstract : The present study contributes to the valorization of three spontaneous medicinal plants ; Juglans regia, Salvadora persica, and Syzygium aromaticum. Three solvents were used, distilled water, ethanol, and methanol, to investigate the antimicrobial activities of these plants at different concentrations: 200, 100, 50, and 25 mg/ml. The various extracts were tested against five pathogenic bacterial strains; Bacillus cereus, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and two fungi: Aspergillus niger and Candida albicans. The results showed that J. regia seems more efficient against the tested strains. Furthermore, J. regia's aqueous extract exhibited significant antibacterial activity, with the highest efficiency of all extracts against S. aureus (mean of diameters of inhibition zone =35 mm). In contrast, the highest inhibition zone for the antifungal activity was observed with the ethanolique extract of S. aromaticum against A. niger (24 mm). Moreover, we assessed their minimal inhibitory concentration (MIC) which values varied between 50 and 3.125 mg/ml. Furthermore, the plant extracts were subjected to phytochemical screening, which detected the presence of flavonoids, polyphenols, saponins, tannins, quinones, and terpenoids. The findings suggest that extracts may include compounds exhibiting therapeutic potential against pathogenic strains, indicating their potential utility as therapeutic agents.