Abstract : Enterobiasis is a gastrointestinal helminthic infection caused by Enterobius vermicularis. An adult human is less susceptible to infection than children. The present study was conducted at the laboratories of Parasitology, Faculty of Natural Sciences and life, University of Setif during the year 2023 to indicate the effect of aqueous and hydro-methanolic extracts of Saponaria officinalis in inhibited hatching and mortality of Enterobius vermicularis eggs in vitro. The efficacy of four extract concentrations (25, 50, 75 and 100 mg/ml) within 6 -48 hours were tested through egg hatch assay and larval motility inside egg. The results were as follows: the mortality was observed 63.3% to 100% in concentrations 75 mg/ml and 100 mg/ml, respectively within 36 -48 hours. Hatching of eggs was observed in control (larval motility inside egg). There was no statistically significant difference between aqueous and hydro-alcoholic extracts (p > 0.05). The result showed that mortality was significant (P<0.05) as concentrations increased from 25-100 mg/ml but mortality was not significant as duration of exposure increased from 24 -48 hours. The result showed that LC50 and LT50 of Saponaria officinalis hydro-methanolic extract, with 54.29 mg/ml and 24.16 hours whiles Saponaria officinalis aqueous extract, with 78.41 mg/ml and 28.66hours. The results revealed that both extract types of Saponaria officinalisinhibited hatching of eggs completely at a concentration highest than 100 mg/ml.