Injuries to the anterior cruciate ligament (ACL) in professional athletes can result in lengthy absences from sports and perhaps endanger their careers. Contact and noncontact are the two primary mechanisms of ACL damage. Noncontact mechanisms are responsible for 60–70% of ACL injuries. The neuromuscular and bio mechanical variables that play a role in this process will be explored in more detail later in the section on injury prevention. To determine mechanism of anterior cruciate ligament injury among football players of Peshawar. A cross sectional study was used to conduct data in Peshawar, Khyber Pakhtunkhwa. Convenient sampling technique was used. A consent form and Questionnaire on anterior cruciate ligament were given to 80 participants, in football academies of Peshawar city. A total of 80 participants had ACL injuries among male football players who responded to the ACL questionnaire. The mean age of the players was 24.38 ± 4.91 years. Most of the injuries were non-contact injuries in football players, ACL injury develop as a noncontact injury during zone of play P=.049, type of ground surface p=.022, playing type p=.021, playing maneuver p=.015 and game intensity p=.048. Maximum players had non-contact ACL injury during game 67/68 and during practice 10/12 out of 80 sample size. In football players, ACL damage can develop as a non-contact injury during zone of play, type of ground surface, playing type, playing maneuver and game intensity. The current findings will aid in determining the causes of anterior cruciate ligament damage in football players, as well as the development of competition-specific rehabilitation and preventive technique.