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[This article belongs to Volume - 56, Issue - 06]

Abstract : Plants are a promising source of bioactive secondary metabolites that could form an alternative to the indiscriminate use of chemical pesticides. Therefore, this study aimed to assess the effect of two aqueous extracts obtained from the leaves of Nerium oleander and Salvia officinalis by maceration method on Drosophila melanogaster Meigen, 1830 (Diptera : Drosophilidae) as biological model. Bio efficacy of extracts was evaluated through different doses (0.5, 1, 5, 10 and 20 μg/larva) by topical application, on the third instar larvae (12 h before pupariation) and the cumulative mortality of immature stage (dead larvae, dead pupae, adult who could not emerge from pupae and partially emerged adult) was determined. Results showed that N. oleander and S. officinalis induced insecticidal activity against D. melanogaster. The inhibition doses (ID) of adult emergence were estimated with ID50values of 6.54 µg/larva for S. officinalis and 3.02 µg/larva for N. oleander. Both extracts showed different levels of insecticidal effect against D. melanogaster and S. officinalis extract was the most active one. These findings suggest that S. officinalis and N. oleander extracts have great potential to be developed as possible natural insecticide for controlling agricultural pests including the harmful Drosophila pest, D. suzukii, however, their field usecan only be ascertained after identification of bioactive compounds and clarifying their mode of action.