Abstract : Thymeleae hirsuta (T. hirsuta) have been traditionally used for various medicinal purposes, including the treatment of inflammation, hypertension, and digestive disorders. This study aims to investigate the effects of decocted (DE) and hydromethanol (HME) extracts from T. hirsuta aerial parts on gastric emptying and intestinal transit in mice, as well as elucidate the underlying mechanisms. Gastric emptying and intestinal transit were assessed in mice following oral administration of DE and HME at different doses (125 mg/Kg, 250 mg/Kg, 500 mg/Kg). Pharmacological substances were used to explore potential synergistic effects; indomethacin, L’Arginine and L-Nitro-N-Arginine (L-NNA). The results indicate that both DE and HME extracts significantly decreased gastric emptying rates compared to the negative control, with (48.68 ± 2.95 %, 38.60 ± 1.67 % and 55.98 ± 2.19 %, respectively) for DE and (66.18 ± 3.7, 51.47 ± 2.7 %, 55.98 ± 2.19 %, respectively) for HME. Mechanistic investigations revealed that both extracts primarily involved the the cyclooxygenase pathway (COX pathway), with no evidence of involvement in the nitrinergic pathway. Furthermore, both extracts induced a significant increase in intestinal transit compared to the negative control, in a dose dependant manner targeting both cyclooxygenase and nitrinergic pathways. The findings suggest that DE and HME extracts from T. hirsuta possess potential therapeutic properties in modulating gastrointestinal motility.