The study on the effect of nitrogen fertilizer fractionation on two wheat varieties, Aba 99 and Al Nour, was conducted in the fields of the College of Agriculture during the autumn agricultural season of 2023 in a mixed soil. The research focused on evaluating some growth and yield indicators of these wheat (Triticum aestivum L.) varieties. The current study focused on two factors: The first factor is two wheat varieties (Nour and Abaa 99) and the second factor is fertilizer levels of 120 kg. ha-1 and symbolized by the symbols (N0, N1, N2), using a factorial experiment based on a randomized complete block design (RCBD) with three replicates. The results indicated that the wheat variety Aba 99 significantly outperformed the Al Nour variety in all studied parameters​​. The study included field emergence rate, field emergence speed, field establishment, dry weight of emerging seedling, number of spikes, number of seeds per spike, weight of 1000 seeds and total yield. The obtained results were 87.16% and 85.27%, 61.72% and 57.15%, 87.16% and 84.99%, 3.84 mg and 3.47 mg 69.94, 331.27 spikesm2 and 302.92 spikesm2, 57.45 seeds spike-1 and 53.74 seeds spike-1, 45.72 g and 41.53 g, 5.43 tons ha-1 and 4.82 tons ha-1 respectively. While the highest results were recorded for the N2 fertilization treatment in all study traits compared to the N1 and N0 treatments that gave unsatisfactory results and the results were 90.54%, 62.82%, 89.15%, 3.98 mg, 359.91 spikes m2, 58.38 grains spike-1, 50.70 g and 6.12 t ha-1. The best interaction observed in the study was between the wheat variety Aba 99 and the nitrogen fertilization treatment N2. This combination resulted in the most favorable outcomes across all the studied characteristics​​.