Numerous studies have been conducted to identify the optimal method for controlling the pest insect Dacus ciliatus on a global scale and prevent extensive plant losses. Chemical pesticides have been discovered to be hazardous, necessitating the search for alternative biocontrol measures. As a non-toxic substitute, nematodes have been advocated as a biocontrol agent by many researchers. Samples of pest insect (larvae and pupae) were infected with commercial and Iraqi native Heterohabditis bacterophora at varying IJs concentrations to compare their impact on Dacus ciliatus control. The test results indicate that a concentration of 100 IJs/µL of Iraqi native Heterohabditis bacterophora produced a killing percentage of 63.33% for larvae and 56.67% for pupae, while a concentration of 100 IJs/µL of commercial Heterohabditis bacterophora. resulted in a killing percentage of 43.33% for larvae and 40.00% for pupae.