Abstract : The objective of this study is to determine the chemical composition and to evaluate the antfungal activity of essential oils from the aerial part of the plant Ferula Tingitana. The extraction of essential oils was carried out by hydrodistillation. The quantitative and qualitative analysis of the essential oil showed a variety of chemical compounds forming the essence of oil, this study identified 22 constituents, representing 97.81% of the essential oil. The main compounds are: Dill ether (25.16%), D-limonene (20.19%), D-Carvone (18.83%), α-phellandrene (16.83%) and O-cymene (5.51%). The results demonstrate an inverse correlation between the percentage inhibition of fungal colony growth rate and increasing concentrations of the essential oil extracted from Ferula Tingitana plants. Inhibition rates of 77.11 ± 0.13%, 65.08 ± 0.64%, and 48.33 ± 0.09% were recorded at concentrations of 15, 10, and 5 µL/Petri dish, respectively. These findings are of significant importance in the field of environmentally friendly pesticide research, as they indicate the potential use of this essential oil as a natural and ecologically safe alternative to conventional fungicides. The study contributes to the development of sustainable agricultural practices by exploring bio-based solutions for crop protection.