Abstract : Malva sylvestris widely used in traditional medicine, is a kind of mallow derived from Malvaceae species and is recognized in Algeria as khoubaiz. This work focuses on the phytochemical study and evaluation of antioxidant, antihemolytic, anti-inflammatory and analgesic activities of flowers methanolic extract of Malva sylvestris. The phytochemical analysis revealed that the extract is rich in polyphenols (74.26±18.70 µg EGA/g extract), and in tannins (18.98±1.33 µg EC/mg extract). The antioxidant activity was investigated by DPPH, ferrous iron chelation, reducing power and AAPH tests. Results showed that methanolic extract is more potent in scavenging DPPH radical with an IC50 of 176.37±20.96 μg/mL and chelating ferrous iron with an IC50 of 195.05± 93.401μg/mL. Similarly, the extract showed relatively high reducing activity with an EC50 of 89.76±6.75 µg/mL and exerts an anti-hemolytic effect against AAPH-induced hemolysis. On the other hand, anti-inflammatory and analgesic activities were determined using ear edema induced by croton oil, air pouch and tortion tests in vivo. Results showed that topical treatment of mice with 2 mg/ear of methanolic extract inhibit edema formation in mice by 76.78%. On the other hand, treatment of mice with 1mg/pocket of extract reduced the migration of leucocytes toward inflammation site by 67.88%. Similarly, oral administration of 200 mg/kg to mice resulted in 72.56% inhibition of acetic acid induced pain. In conclusion, flowers methanolic extract of Malva sylvestris has antioxidant and anti-inflammatory properties which supports the traditional use to treat certain disorders linked to inflammation and oxidative stress.